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2003:
STARE I PRIZNATE SORTE U EKOLOŠKOJ PROIZVODNJI POVRĆA

UTJECAJ ROKOVA UZGOJA I BERBE NA KVALITATIVNA SVOJSTVA KINESKE RAŠTIKE (Brassica rapa L. ssp. chinensis (L.) Hanelt

UTJECAJ MIKORIZE I RAZLIČITO OBOJENIH PE FILMOVA NA PRINOS SLATKOG KRUMPIRA - BATATA (Ipomea batatas L.)

INFLUENCE OF SUBSTRATE COMPOSITION ON THE QUANTITY AND QUALITY OF PLEUROTUS MUSHROOMS YIELD

HIDROPONIJSKI UZGOJ PAPRIKE (Capsicum annuum L.) NA KAMENOJ VUNI

MORPHO - ANATOMICAL CHANGES PRODUCED BY THE PHYTOPHTHORA INFESTANS (MONT.) DE BY. AT THE TOMATO CROP IN GREENHOUSE

NEKE KARAKTERISTIKE SLOVENSKIH SORTI POVRĆA ZA EKOLOŠKU PROIZVODNJU

THE DROOPING IRRIGATION AND THE WATER CONSUMPTION IN THE CASE OF SOLARIUM CULTIVATED CUCUMBERS

ANALIZA PROFITABILNOSTI PROIZVODNJE RANOG KUPUSA

PRINOS I SKLADIŠNA SPOSOBNOST SORATA LUKA

FIELD RESISTANCE OF SEVEN DIFFERENT LETTUCE CULTIVARS ON THE OCCURRENCE OF LETTUCE DOWNY MILDEW (Bremia lactucae Regel)

YIELD AND QUALITY OF FORCED CHICORY (Chicorium intybus L.) cv. 'Goriški'

MINERALNI SASTAV RADIČA (Cichorium intybus var. foliosum L. ) PRI RAZLIČITIM GNOJIDBAMA

UTJECAJ STAROSTI PRESADNICA I KULTIVARA NA KOMPONENTE PRINOSA TIKVICE

THE NEW ADMITED DOMESTIC AND INTRODUCED VARIETIES OF TOMATOES

UTJECAJ SUSTAVA PROIZVODNJE I MIKORIZE NA VEGETATIVNI RAST I PRINOS RAJČICE U POSTRNOM ROKU UZGOJA

THE EFFECT OF DIFFERENT TYPES OF FERTILIZATION AND PLANTS DENSITY ON TOMATOES CULTIVATED IN GREENHOUSE ON ORGANIC SUBSTRATUM

UZGOJ RAJČICE NA INERTNIM SUPSTRATIMA

SUVREMENI KOMUNIKACIJSKI SUSTAVI U FUNKCIJI RAZVOJA HRVATSKE POVRĆARSKE PROIZVODNJE

IZBOR KULTIVARA RAJČICE ZA HIDROPONSKI UZGOJ

KAKVOĆA PLODOVA RAJČICE UZGOJENIH NA KAMENOJ VUNI

EFIKASNOST FOLIJARNO DODANOG Ca U SPRJEČAVANJU POJAVE VRŠNE TRULEŽI PLODA PAPRIKE

INTRODUKCIJA KULTIVARA LUBENICE U DALMATINSKA KRŠKA POLJA

NAVODNJAVANJE ZASLANJENOM VODOM U UZGOJU LUBENICE

PROMJENE SADRŽAJA ŠEĆERA TIJEKOM MLIJEČNE ZRIOBE GENOTIPOVA KUKURUZA ŠEĆERCA

PREZENTIRANI I OBJAVLJENI RADOVI iZ POVRĆARSTVA
pošalji upit
Zbornik sažetaka 38. znanstvenog skupa hrvatskih agronoma 2003. godine - OPATIJA
REACTION OF CAPSICUM SPECIES, HYBRIDS AND BREEDING LINES TO OBUDA PEPPER TOBAMOVIRUS (SYN: TOMATO MOSAIC TOBAMOVIRUS)

Gabriella KAZINCZI, János KOVÁCS, András Péter TAKÁCS, József HORVÁTH

University of Veszprém, Georgikon Faculty of Agricultural Sciences, Keszthely, Hungary (E-mail: H11895HOR@ELLA.HU)

Introduction

Peppers (Capsicum spp.) originating from Mexico, Southern Peru and Bolivia are grown world-wide under various environmental and climatic conditions. Last decades pepper growing under glasshouse and tunnels achieved considerable results in Hungary. The consumption of our traditionally national product - sweet pepper - increases not only in Hungary but also abroad. Besides sweet pepper, red spice one is also an important national product in Hungary.

Among pathogens, viruses are one of the major limiting factors in successful pepper growing (Tiznado and Carrillo 2002). In Hungary 13 viruses have been isolated from pepper plants so far (Kazinczi et al. 2001). The extremely stable, mechanically transmissible tobamoviruses are found to be the major problems under glasshouse and tunnels, while the dominance of the aphid-borne cucumo- poty- and alfamoviruses were demonstrated in the open fields (Kiss 1996, Gáborjányi et al. 1997, Kálmán et al. 2000). Among tobamoviruses, a new one: Obuda pepper tobamovirus (ObPV) [Syn: Ob strain of tomato mosaic tobamovirus, (ToMV-Ob)] has been appeared in the 1980s, which has broken the resistance of pepper varieties, containing the L1 gene (Tóbiás et al. 1982, Csilléry et al. 1983). Resistance breeding is the most effective method against virus diseases. Since the 1980s, intensive research work started in order to study the response of different pepper varieties, breeding lines and wild Capsicum species to viruses in Hungary. Out of them new sources of resistance have been found (Horváth 1986, Kazinczi et al. 2001).

The objective of this study was to examine the reaction of 25 Capsicum species, hybrids and breeding lines to ObPV.

Materials and methods

Seeds of 25 Capsicum species, hybrids and breeding lines were sown in sterilized boxes in virological glasshouse. The seedlings were planted in plastic pots containing a soil mixture of sand (pH 6.96, humus% 0.27):peat (pH 6.78, humus% 9.98) 1:3. Seven plants of each species, +hybrids and breeding lines were mechanically inoculated with the Obuda pepper tobamovirus (ObPV). The inoculated plants were symptomatologically checked for virus infection. Five weeks after inoculation the samples were tested using direct double-antibody sandwich ELISA (DAS ELISA) serological method. Substrate absorbance was measured 20 minutes after adding the substrate at 405 nm wavelength on Labsystems Multiskan RC ELISA Reader. Test samples were considered positive, if their absorbance values exceeded twice those of the healthy control samples. To confirm the results of symptomatology and serology, back inoculation was also carried out to Nicotiana tabacum 'Xanthi-nc' (local host of ObPV) and 'Samsun' (systemic host of ObPV) as indicator plants.

Results and discussion

No breeding materials showing extreme resistance (immunity) have been found. Out of the Capsicum species, hybrids and breeding lines 12 proved to be susceptible to ObPV. Both local and systemic symptoms have been occurred and extinction values of the samples were similar, than those of the positive control. 13 samples showed hypersensitive reaction. Necrotic lesions have been developed on the infected leaves 2-4 days after inoculation, and later the infected leaves dropped, preventing the spreading of the virus inside the whole plants (Table 1). Plant resistance to virus infection expressed in the form of restricted movement of the virus has been described for many other host-virus relations (Nelson et al. 1993, Schaad and Carrington 1996, Derrick and Barker 1997).

Conclusion

It is concluded that 18/98F1, 18/98 F2, 4/99 F3, V-14 21/98, Capsicum testiculatum, C. chinense PI. 159234, C. annuum 'Criollo de Morelos', 255 Pritamin line, 5/96 C. testiculatum x Botond F2, 47/87 F7, 265 Red Chili, 411 C. annuum x C. frutescens and 419 Fűszer USA breeding materials could be used for breeding of resistance to ObPV. Regarding that mixed infections occur commonly in nature it could be worth to examine the reaction of breeding materials to other economically important viruses.

Table 1. Reaction of Capsicum species,hybrids and breeding lines to ObPV

 Symptoms (local/systemic)*AbsorbanceBack inoculationType of host-virus relation**
Capsicum baccatumNl/Mo1.597+S
C.baccatum var. pendulumNl/Mo1.511+S
C. testiculatumNl/-0.233-HR
C. chinense PI. 159234Nl/-0.189-HR
C. chacoenseNl/Mo1.307+S
529 C. annuum 'Perennial'-/Mo1.664+S
C .annuum 'Criollo de Morelos'Nl/-0.204-HR
255 Pritamin lineNl/-0.169-HR
5/96 C. testiculatum x Botond F2Nl/-0.145-HR
47/87 F7Nl/-0.194-HR
413 C. frutescens x C. annuumNl/Tn,D1.410+S
265 Red chiliNl/-0.212-HR
420 Fehér alma-/Mo,Led1.359+S
40/85 F6 Csípős fehérNl/Mo, Led1.094+S
411 C. annuum x C. frutescensNl/-0.228-HR
416 Santa Fe GrandeNl/Mo, Ye, Led1.340+S
419 Fűszer USANl/-0.206-HR
18/998 F1 HattyúNl/-0,187-HR
18/98 F1 SzépNl/-0.202-HR
VI-43 GypsiNl/Mo1.212+S
4/99 F3Nl/-0.199-HR
V-33Nl/Mo, Bli, Led0.998+S
35/98 F1Nl/Mo, Bli, Led1.420+S
5/99 F2Nl/Led, Mo, Ye1.397+S
V-14 21/98Nl/-0.198-HR
Positive control 1.521  
Negative control 0.191  
*Nl, necrotic lesions; Mo, mosaic; Tn, top necrosis; D, death of the plant; Led, leaf deformation; Ye, yellowing; Bli, blistering; -, symptomless **S, susceptible; HR, hypersensitive reaction

Acknowledgements

We would like to thank the Office for Academy Research Groups Attached to Universities and Other Institutes for their financial support.

Literature
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  2. Derrick P. and Barker H. (1997). Short and long distance spread of potato leafroll luteovirus: effect of host genes and transgenes conferring resistance to virus accumulation in potato. J. Gen. Virol. 78: 243-251.

  3. Gáborjányi R., Pogány M. and Horváth J. (1997). Role of viruses in pepper decline. Növényvédelem 33: 181-185.

  4. Horváth J. (1986). Compatible and incompatible relations between Capsicum species and viruses. I. Review. Acta Phytopath. Hung. 21: 35-50.

  5. Kálmán D., Kassai T., Tornyai T. and Gáborjányi R. (2000). Pepper mild mottle tobamovirus: new pepper pathogen in Hungary. Növényvédelem 36: 613-618.

  6. Kazinczi G, Horváth J. and Gáborjányi R.(2001). Some aspects of pepper virus research. Acta Phytopath. et Entomol. Hung. 36: 329-347.

  7. Kiss E. (1996).Virus diseases of greenhouse pepper in South-Hungary. International Workshop on Biological and Integrated Pest Management in Greenhouse Pepper. Hódmezővásárhely (Hungary) 1996. pp. 119-131.

  8. Nelson R., Li G., Hodgson R., Beachy R. and Shintaku M. (1993). Impeded phloem-dependent accumulation of the masked strain of tobacco mosaic virus. MPMI 6: 45-54.

  9. Schaad M. and Carrington J. (1996). Suppression of long distance movement of tobacco etch virus in a nonsusceptible host. J. Gen. Virol. 70: 2556-2561.

  10. Tiznado G. and Carrillo M. (2002). Past and present status of viruses affecting chili pepper in Mexico. 16th Internat. Pepper Conf. Tampico (Mexico) 2002. p.8.

  11. Tóbiás I., Rast A. and Maat D. (1982). Tobamoviruses of pepper, eggplant and tobacco: Comparative host relations and serological relationships. Neth. J.Pl. Path. 88: 257-268.


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